Multiplex SNP Assays

The SNPWave technology can detect several to 30 SNP loci simultaneously. A multiplexed SNP detection assay reduces the costs per SNP datapoint considerably compared to monoplex SNP detection assays. SNPWave is therefore most suitable fro high-throughput diagnostic purposes.

How it works

Figure 1: Basic principle of SNPWave Multiplex allele-discrimination based on ligation using probes with unique stuffer (green), differing 2 basepairs (bp) between alleles of a SNP locus and 3 bp between SNP loci.

Adobe Systems

Figure 2: Example of a SNPWave assay. Reconstruction of a gel image of SNPWave reaction products from 16 individuals. Each column shows a different fingerprint that represents a specific haplogroup. (Column x represent an example fingerprint as a demonstration of a forensic case matching profile #13).


  • Genotyping;
  • variety determination;
  • allelic variation detection;
  • diagnostic assays;
  • micro-organism detection;
  • Mixed population detection.


  • Multiplex allele discrimination;
  • full design based on single pair of primers per allele;
  • Detection on several platforms.


  • Design based on a set of 10-30 SNP’s;
  • delivery of fully tested SNPWave assay within 3 months;
  • 10,000 reactions are delivered at the first order.


  • Multi marker assay in vegetables
  • Multi marker assays in field crops
  • Y-Chromosome
  • Coeleac Disease diagnostic assay
More info

Herco van Liere

Vice-President Business Development
T +31 (0)317 466 866